What is a Poly-L-Lysine Slide used for?

A Poly-L-Lysine Slide is primarily used in laboratories for cell culture applications. The positively charged lysine molecules on the slide surface enhance cell adherence, making it ideal for growing and studying various cell types under a microscope. This slide is particularly useful for researchers looking to analyze cellular behaviors, perform immunofluorescence, or conduct microscopy of cultured cells.

How do I prepare a Poly-L-Lysine Slide for use?

To prepare a Poly-L-Lysine Slide, first ensure the surface is clean by rinsing it with distilled water. For optimal cell attachment, some protocols recommend incubating the slide with a specific cell suspension buffer before adding the cells. This step helps maintain the hydrophilic nature of the surface, facilitating better cell adhesion and growth during your experiments.

Can Poly-L-Lysine Slides be reused?

Poly-L-Lysine Slides are generally designed for single-use to maintain sterility and prevent contamination. However, if properly cleaned and sterilized using appropriate methods such as autoclaving, they may be reused for specific laboratory applications. Always consider the type of cell being cultured and the experiment design before reusing these slides.

Are Poly-L-Lysine Slides suitable for all types of cells?

While Poly-L-Lysine Slides are effective for many adherent cell types, such as fibroblasts and neuronal cells, they may not be suitable for suspension cells that do not adhere to surfaces. If you're working with such cells, it's advisable to consider alternative substrates that are designed for their specific needs. Conduct prior tests to confirm cell compatibility with the slide.

What are the storage conditions for Poly-L-Lysine Slides?

Poly-L-Lysine Slides should be stored in a cool, dry place away from direct sunlight. Keeping them in their original packaging until ready for use helps maintain sterility and effectiveness. Avoid exposing the slides to extreme temperatures or moisture, as these can degrade the chemical properties of the Poly-L-Lysine coating.

Can I use Poly-L-Lysine Slides for immunohistochemistry?

Yes, Poly-L-Lysine Slides are commonly used for immunohistochemistry applications. Their ability to enhance cell attachment allows for better processing and staining of cells, making them ideal for this type of analysis. For optimal results, ensure the cells are evenly distributed and the proper fixation and staining protocols are followed to visualize specific proteins effectively.

What are the benefits of using Poly-L-Lysine Slides?

Poly-L-Lysine Slides offer several benefits, including enhanced cell adhesion and improved imaging quality. Their positive charge promotes strong binding with negatively charged cell membranes, which is critical for growing cells effectively on glass surfaces. This improved adhesion leads to more reliable experimental outcomes when studying cell morphology or cellular responses in research.

Are there any limitations to using Poly-L-Lysine Slides?

One limitation of Poly-L-Lysine Slides is that they may not support the growth of all cell types, particularly non-adherent or suspension cells. Additionally, the concentration of Poly-L-Lysine may need optimization depending on the specific cell line used. It is essential to conduct preliminary tests to determine which concentrations work best for particular applications.

What is the typical size of a Poly-L-Lysine Slide?

Poly-L-Lysine Slides typically come in standard microscope slide sizes, generally measuring 75 mm x 25 mm. This size is ideal for most laboratory applications, providing sufficient surface area for cell culture while fitting standard microscopes for imaging. If specific dimensions are needed, verify with the manufacturer for custom options.

Where can I buy Poly-L-Lysine Slide, 20/BX in Serbia?

You can purchase Poly-L-Lysine Slide, 20/BX from Ubuy in Serbia. Ubuy offers a convenient platform for accessing a variety of laboratory supplies, including Poly-L-Lysine Slides, ensuring you can find the products you need for your research quickly and easily.

Како купити Poly-L-Lysine Slide, 20/BX на мрежи од Убуи-а?

Лако је куповати Poly-L-Lysine Slide, 20/BX на мрежи од Убуи-а.. Морате само да потражите производ, изаберете начин испоруке приликом одјављивања и да га испоручите на вашу локацију.

Да ли је Poly-L-Lysine Slide, 20/BX доступан за онлајн куповину у Serbia?

Да, у Убуи Serbia овај производ је доступан за куповину по разумној цени.. Poly-L-Lysine Slide, 20/BX није доступан локално, али можете нам веровати у наше услуге експресне испоруке.

Колико времена је потребно да се производ добије након наручивања?

Време испоруке вашег нарученог производа варира у зависности од тога шта сте наручили и начина испоруке који сте изабрали.. Процењено време испоруке се помиње током процеса плаћања, па будите безбрижни док купујете.

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These slides yield a dense layer of amine groups for initial ionic attachment of DNA.

Electron Microscopy Sciences

artikal br.: 92620453

Poly-L-Lysine Slide, 20/BX

artikal br.: 92620453

RSD 36149

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These slides yield a dense layer of amine groups for initial ionic attachment of DNA.

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What Stands Out

High Purity

Made from high-purity Poly-L-Lysine, ensuring optimal cell adhesion and growth for reliable experimental outcomes.

Versatile Applications

Ideal for diverse laboratory applications, including tissue culture and immunohistochemistry, catering to a wide range of scientific research needs.

Cost-Effective Packaging

Offered in convenient 20-slide boxes, providing an economical solution for labs in need of multiple samples without sacrificing quality.

Детаљи о производу

Item Weight

1.5 lbs (680 grams)

Who Should Buy?

Researchers
Ideal for scientists conducting cell culture experiments requiring enhanced adhesion of cells to slides for microscopy.
Educators
Useful for teaching labs in biology to demonstrate cell attachment and growth on coated surfaces effectively.
Histologists
Beneficial for histopathology labs needing a reliable substrate for tissue samples to achieve optimal slide preparation.

Casual Users
Not suitable for non-professionals without laboratory experience who may misuse or mishandle the product poorly.

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Pitanje: Како купити Poly-L-Lysine Slide, 20/BX на мрежи од Убуи-а?

Odgovor: Лако је куповати Poly-L-Lysine Slide, 20/BX на мрежи од Убуи-а.. Морате само да потражите производ, изаберете начин испоруке приликом одјављивања и да га испоручите на вашу локацију.
Pitanje: Да ли је Poly-L-Lysine Slide, 20/BX доступан за онлајн куповину у Serbia?

Odgovor: Да, у Убуи Serbia овај производ је доступан за куповину по разумној цени.. Poly-L-Lysine Slide, 20/BX није доступан локално, али можете нам веровати у наше услуге експресне испоруке.
Pitanje: Колико времена је потребно да се производ добије након наручивања?

Odgovor: Време испоруке вашег нарученог производа варира у зависности од тога шта сте наручили и начина испоруке који сте изабрали.. Процењено време испоруке се помиње током процеса плаћања, па будите безбрижни док купујете.

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RSD 36149

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Карактеристике и предности

Made with premium low fluorescence glass for superior performance.
Features a uniform coating of poly-L-lysine for optimal DNA attachment.
Allows for covalent DNA attachment via baking or UV irradiation.
Tested for background uniformity before shipping for quality assurance.
Ready for use upon arrival - no pre-washing required.
Comes as a secure package containing 20 slides, vacuum-sealed for freshness.

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Poly-L-Lysine Slide, 20/BX

RSD 36149

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100% Original Products

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What Stands Out

Детаљи о производу

Who Should Buy?

OPIS PROIZVODA

Питања и одговори купаца

Electron Microscopy Sciences Microscope Sample Slides Editorial Review

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RSD 36149

Карактеристике и предности

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